Histone F1. Purification and phosphorus content
نویسندگان
چکیده
منابع مشابه
Histone F1 of Tetrahymena Macronuclei
Histone fraction F1 has been isolated and purified from macronuclei of the ciliated protozoan, Tetrahymena pyriformis. In many respects, Tetrahymena F1 is similar to that of other organisms. It is the only Tetrahymena histone soluble in 5% perchloric acid or 5% trichloroacetic acid, has a higher molecular weight than any other Tetrahymena histone, is the histone most easily dissociated from Tet...
متن کاملVariations in the phosphate content of histone F1 in normal and irradiated tissues.
1. The capacity of the histone-DNA complex to act as a template for RNA synthesis is increased by phosphorylation of histone F1. 2. In regenerating liver, DNA synthesis is preceded by phosphorylation of histone F1. 3. Exposure to ionizing radiation in vivo decreases and delays the phosphorylation of histone F1 in regenerating liver, and decreases it in the hyperplastic kidney. 4. Histone F1 is ...
متن کاملInteraction of f1 histone with superhelical DNA.
The superhelicity of double-stranded, closed circular SV40 DNA was altered by the addition of various amounts of ethidium bromide. The interaction of f1 histone with the series of molecules of various superhelicities was studied. The extent of interaction increases with increasing superhelicity regardless of whether it is of the positive or negative sense. The interaction of f1 histone with sup...
متن کاملImmunological relatedness of thymus and liver F1 histone subfracions.
Antibodies to chromatographically purified rat thymus Fl histone subfractions were induced in rabbits by immunization with histone-RNA complexes. The immunological relatedness between each of the rat thymus Fl subfractions and subfractions derived chromatographically from calf thymus and rat liver Fl was determined by quantitative microcomplement fixation. The various rat thymus peaks elicited ...
متن کاملAnalysis of Histone Exchange during Chromatin Purification.
Central to the study of chromosome biology are techniques that permit the purification of small chromatin sections for analysis of associated DNA and proteins, including histones. Chromatin purification protocols vary greatly in the extent of chemical cross-linking used to prevent protein dissociation/re-association during isolation. Particularly for genome-wide analyses, chromatin purification...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1970
ISSN: 0306-3283
DOI: 10.1042/bj1170157